Figure 5

Deletion of calmodulin-binding site on TRPV1 does not affect AKAP150-mediated TRPV1 activity. (A) Representative calcium-imaging traces of CAP-stimulated (50 nM, 30 sec) intracellular Ca²⁺ accumulation from CHO cells expressing AKAP150 and TRPV1, TRPV1ΔN CaM or TRPV1ΔC CaM following vehicle (Veh, H₂0) or 8-Br-cAMP (8-Br-cAMP, 10 μM, 30 sec) pre-treatment. (B) Data represented in(A) are quantified as ΔF340/F380, mean ± SEM shown, n shown for each treatment/transfection paradigm. Black asterisks indicate significance between indicated groups. White asterisks indicate significance between marked treatment group and AKAP150 and TRPV1 expressing treatment groups (Veh-treated AKAP150 + TRPV1 ΔC CaM v. Veh-treated AKAP150 + TRPV1, for example). (C) Data in B are transformed to illustrate percent sensitization following 8-Br-cAMP pre-treatment. Significance determined by one-way ANOVA with Bonferroni correction, *p < 0.05, ***p < 0.001.