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Figure 7 | Molecular Pain

Figure 7

From: AKAP150-mediated TRPV1 sensitization is disrupted by calcium/calmodulin

Figure 7

Calmodulin co-expression decreases TRPV1 and AKAP150 association. (A) Co-immunoprecipitation and Western blot analysis of TRPV1 and AKAP150 from CHO cells co-expressing wt CaM or DN CaM. (B) Quantified densitometry of co-immunoprecipitation results normalized to total TRPV1 expression. (C) CAP (100 nM, 30 sec) -current responses (100 nM, 30 sec) from CHO cells expressing AKAP150 and TRPV1 with empty vector pcDNA3, wt CaM, or DN CaM, following vehicle (Veh, H20, gray bars) or 8-Br (8-Br-cAMP, 10 μm, 30 sec, black bars) pre-treatment, n = 6-9 per transfection/treatment paradigm. Black asterisks indicate significance between indicated groups. Significance determined by one-way ANOVA with Bonferroni correction, NS = no significance, *p < 0.05, ***p < 0.001. (D-F) Representative current traces from CHO cells expressing AKAP150, TRPV1, and empty vector (D), wtCaM (E), or DNCaM (F).

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