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Figure 4 | Molecular Pain

Figure 4

From: Monosodium iodoacetate-induced joint pain is associated with increased phosphorylation of mitogen activated protein kinases in the rat spinal cord

Figure 4

Expression of spinal pERK1/2 and p-p38 in neuronal and glial cells of MIA-OA rats. Immunohistochemical double-labeling with the fluorescence dyes Cy2 (red; pERK1.2 or p-p38) and Cy3 (green; NeuN, GFAP, or OX-42) was conducted in lumbar spinal cord sections where orange-yellow fluorescence represents colocalization (white arrow). In the dorsal horn of OA rats 3-wk following MIA, double labeling with anti pERK1/2 (A-C) revealed colocalization with anti-NeuN/neuron (A), but not anti-GFAP/astrocyte (B) or anti-OX-42/microglia (C). Double labeling with anti-p-p38 (D-F) revealed colocalization with anti-NeuN (D), but limited to a subpopulation of small-diameter neurons. Colocalization of anti p-p38 in glial cells was not observed with anti-GFAP (E), but was observed with anti-OX-42 (F).

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