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Figure 9 | Molecular Pain

Figure 9

From: Glial TNFα in the spinal cord regulates neuropathic pain induced by HIV gp120 application in rats

Figure 9

The antinociceptive effects of recombinant soluble TNF receptor or TNFα knockdown with TNFα siRNA in rats treated with gp120 application. (A) Rats received an intrathecal injection of recombinant soluble TNF receptor I or vehicle. Administration of recombinant soluble TNF receptor I, but not vehicle, reversed the mechanical threshold, F (1, 11) = 10.808, p = 0.007, n = 6-7, General linear model, repeated measure, SPSS. (B) The downregulation of TNFα in the cultured glial cell line by TNFα siRNA. The glial cells were pretreated with the TNFα siRNA or the mismatch RNA, and then stimulated with LPS. TNFα siRNA significantly lowered the TNFα expression in the cells treated with LPS, *p < 0.01, t test, n = 3. (C) The effect of intrathecal TNFα siRNA on the mechanical threshold in rats treated with gp120 application. Administration of TNFα siRNA, but not the mismatch RNA, reversed the mechanical threshold, F (1, 12) = 20.293, p = 0.001, n = 7, General linear model, repeated measure, SPSS.

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