Colocalization of TRPA1 and IB4, Ret or GFR αs in DRG neurons. (A): Double immunostaining of TRPA1 (green) and IB4 (red) showed almost all of TRPA1 were expressed in IB4-labeled neurons of the naïve rat DRG. (B-E): Colocalization of TRPA1 mRNA (brown cells) and Ret (B), GFR α1 (C), GFR α2 (D), and GFR α3 (E) mRNAs (clusters of silver grains) using double ISHH with 35S-labeled antisense Ret and GFR αs probes combined with TRPA1 DIG-labeled probe. The bright field and dark field photographs are merged. Arrows indicate examples of double-labeled neurons. Scale bar = 100 μm in A, = 50 μm in B-D.