Figure 7
From: Transcription factors Sp1 and Sp4 regulate TRPV1 gene expression in rat sensory neurons
Over-expression of transcription factor Sp1 and Sp4 mRNA increase endogenous TRPV1 mRNA in cultured DRG neurons. (A) Measurement of endogenous levels of rat Sp1 mRNA in cultured DRG neurons following transfection with empty/PN3 vector (left). Additional expression of an equivalent amount of human form of Sp1 mRNA was achieved following transfection with hSp1/PN3. (B) Endogenous TRPV1 mRNA levels were increased following over-expression of hSp1 in cultured DRG neurons (**) p < 0.005. (C) Measurement of control levels of rat Sp4 mRNA in cultured DRG neurons following transfection with empty/PN3 vector (left). Additional expression of an equivalent amount of human form of Sp4 mRNA was also achieved following transfection with hSp4/PN3. (D) Endogenous TRPV1 mRNA levels increased following over-expression of hSp4 in cultured DRG neurons (*). Error bars SEM (n = 3) triplicate measures. Two tailed unpaired t-test. Significance: (p < 0.05). Ct threshold values were derived from quantitative RT-PCR amplification of cultured rat DRG neuron RNA - see Methods.