Figure 3

Anti-nociceptive effect of intrathecal FC and U0126. (A) The von Frey threshold in group FC was significantly increased 4-12 h post FC intrathecal injection compared with that in group FC vehicle sham (Figure 3A). All data are expressed as means ± SEM. *P < 0.05, ** P < 0.01 vs. group FC vehicle sham. ## P < 0.01 vs. group FC model sham. (B) Three doses of U0126 (0.1, 1.0 and 10 μg) were compared (Figure 3B). 1 μg (group U0126-1.0)and 10 μg (group U0126-10) U0126 significantly attenuated the mechanical allodynia induced by bone cancer pain, but 0.1 μg (group U0126-0.1) U0126 had no effect. 1 μg U0126 (group U0126-1.0) attenuated the mechanical allodynia for 6 h after administration, while 10 μg U0126 (group U0126-10) attenuated the mechanical allodynia for 9 h after treatment. This suggested that intrathecal injection of 10 μg U0126 may relieve pain, for up to 9 h after administration. *P < 0.05, ** P < 0.01 vs. group U0126 vehicle sham. ## P < 0.01 vs. group U0126 model sham.