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Figure 6 | Molecular Pain

Figure 6

From: Cancer-induced bone pain sequentially activates the ERK/MAPK pathway in different cell types in the rat spinal cord

Figure 6

Sequent activation of p-ERK in different cell types respectively. On day 3 after carcinoma cell inoculation, moderate p-ERK immunoreactive (IR) neurons (Figure 6B) were found in ipsilateral spinal cord which were much more than that of the control (Figure 6A-aa). The p-ERK is colocalized with NeuN (Figure 6B-bb, green), and the double-labeling cells (Figure 6 B-bbb, yellow) were mainly located in the superificial dorsal horn (laminae I-II) of the ipsilateral L4-5 spinal cord. The relative peak of p-ERK-IR microglia was on the day 6 after carcinoma cell inoculation. The microglia p-ERK activation (Figure 6C-cccc, yellow) was widespread in the dorsal and ventral horns. Almost all p-ERK-IR cells (Figure 6C-cc, red) in the L4-5 spinal cord expressed OX-42 (Figure 6C-ccc, green) which were much more than that of the control (Figure 6A-aaa). Moreover, on day 12, double staining indicated that the p-ERK (Figure 6D-dd, red) was predominantly localized in GFAP-IR astrocytes (Figure 6D-ddd, green).

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