Figure 3

Resveratrol treatment decreases p35 and Egr-1 mRNA levels and blocks the effects of TNF-α in PC12 cells. A) Real-time RT-PCR analysis of p35 mRNA levels normalized against S29. Total RNA was obtained from PC12 cells treated with resveratrol (25 μM) for 0, 6, 15 and 24 h. After reverse transcription, we conducted real-time PCR with specific primers for p35 and S29. B) PC12 cells were treated with TNF-α (50 ng/ml), and TNF-α (50 ng/ml) plus resveratrol (25 μM) during a 3 h period. Resveratrol blocked the increase in p35 mRNA expression induced by TNF-α.C) Real-time RT-PCR analysis of Egr-1 mRNA levels normalized against S29. Total RNA was obtained from PC12 cells treated with resveratrol (25 μM) for 0, 1, 2 and 4 h. After reverse transcription, we carried out real-time PCR with specific primers for Egr-1 and S29. D) PC12 cells were treated with TNF-α (50 ng/ml), and TNF-α (50 ng/ml) plus resveratrol (25 μM) during a 1 h period. Resveratrol blocked the increase in Egr1 mRNA expression induced by TNF-α Bars are the mean and SEM of 3 independent experiments measured in triplicate. *p < 0.05, **p<0.01 (Bonferroni test after ANOVA). #p <0.05 (Bonferroni test after ANOVA), # mean value for TNF-α treatment as compared to mean value for TNF-α plus resveratrol treatment.