Figure 4

Resveratrol treatment decreases p35 protein levels and Cdk5 kinase activity in PC12 cells and rat DRG neuronal cultures. A) Representative Western blots analysis and Cdk5 kinase activity of protein from PC12 cells treated with resveratrol (Resv 25 μM) and untreated cells (Cont) during 24 h. Resveratrol treatment decreased p35 protein levels, and Cdk5 kinase activity. The levels of Cdk5 were unchanged and α-tubulin western blot was used as a loading control. B) Quantification of resveratrol treatment (25 μM) significantly decreased p35 protein expression in PC12 cells. C) Quantification of resveratrol treatment (25 μM) significantly decreased Cdk5 kinase activity in PC12 cells. D) Representative Western blots analysis and Cdk5 kinase activity of protein from rat DRG neuronal culture treated with resveratrol (Resv 25 μM) and untreated (Cont) during 24 h. Values are shown as mean and SEM of 3 independent experiments. * p < 0.05 by using t-test.