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Figure 2 | Molecular Pain

Figure 2

From: Reduced spinal microglial activation and neuropathic pain after nerve injury in mice lacking all three nitric oxide synthases

Figure 2

Spinal nerve injury-induced tactile allodynia and microglial activation in iNOS-/- and n/i/eNOS-/- mice. PWTs (A; ipsilateral hindpaw, B; contralateral hindpaw) of WT mice (n = 9), iNOS-/- mice (n = 8) and n/i/eNOS-/-mice (n = 9) before (0) and 1, 3, 7, 10 and 14 days after fifth lumbar spinal nerve injury. All data are presented as means ± SEM. Statistical analysis were determined by two-way repeated ANOVA with Tukey's test, *P < 0.05, **P < 0.01, *** P < 0.001 vs. WT mice. (C) Photomicrographs show immunostaining with Iba1, a marker of microglia, in the fifth lumbar spinal dorsal horn of WT mice (a-c), iNOS-/-mice (d-f) and n/i/eNOS-/-mice (g-i) 14 days after nerve injury. Scale bars: 200 μm (b, e, h) and 20 μm (a, c, d, f, g, i) (D) The number of Iba1+microglia in the spinal dorsal horn (n = 4 animals/group). All data are presented as means ± SEM. Statistical analysis were determined by two-way ANOVA with Tukey's test, *P < 0.05, ***P < 0.001 vs. contralateral, #P < 0.05, ##P < 0.01.

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Molecular Pain

ISSN: 1744-8069

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