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Figure 3 | Molecular Pain

Figure 3

From: Reduced spinal microglial activation and neuropathic pain after nerve injury in mice lacking all three nitric oxide synthases

Figure 3

Reduction in the amount of p-SFKs in iNOS-/- and n/i/eNOS-/- mice, and changes in IL-6 and TLR4 mRNA expression levels in n/i/eNOS-/- mice. (A) Photomicrographs show immunostaining for phosphorylated-Src family kinases (p-SFKs) in the fifth lumbar spinal dorsal horn of WT mice (a), iNOS-/-mice (b) and n/i/eNOS-/-mice (c) 14 days after spinal nerve injury. Scale bar: 200 μm. (B) Analysis of p-SFKs immunoreactivity in the spinal dorsal horn of WT mice, iNOS-/-mice and n/i/eNOS-/-mice (n = 3 animals/group). (C, D) Levels of interleukin-6 (IL-6) and toll-like receptor 4 (TLR4) mRNA expression. Expression of IL-6 and TLR4 were significantly increased in the ipsilateral spinal cord when compared with the contralateral spinal cord in WT mice (n = 5) 14 days after nerve injury. In n/i/eNOS-/-mice (n = 4), IL-6 (C) and TLR4 (D) mRNA expression levels were significantly attenuated when compared with WT mice. All data are presented as a mean ± SEM. Statistical analysis was determined by one-way ANOVA with Tukey's test, **P < 0.01, #P < 0.05.

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Molecular Pain

ISSN: 1744-8069

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