Figure 5
From: Lentiviral gene transfer into the dorsal root ganglion of adult rats
Immunoblotting analysis of EGFP expression in ipsilateral DRG. Results in two ipsilateral DRGs (Inj. DRG in lanes 4 and 5) following VSV-G coated LV2 EF1α-EGFP vector, as well as a non-injected DRG (lane 3). Lane 2 is the homogenate from HeLa cells transduced by EF1α-EGFP vector as an EGFP positive control, and lane 1 shows protein standards (MagicMark, Invitrogen). Arrowheads point to the expected size bands for EGFP (top panel), and α-tubulin (bottom panel) as loading control. The additional band in the bottom panel (asterisk) indicates an additional tubulin isoform in DRG.