Generation of Advillin-Cre transgenic mice. A. Schematic representation of ET-recombination based insertion of a NLS-Cre gene cassette into the coding region of a BAC containing the mouse Advillin gene locus. The cassette was recombined into the locus immediately after the start codon of exon 2. After purification, the final 177 kb construct was injected into the pronucleus or used for intracytoplasmic sperm injection. HA: homology arms for recombination; NLS: SV-40-T nuclear localization signal; Cre: Cre gene; V5: V5 epitope tag; PA: SV-40 polyadenylation signal; EM7-KAN: kanamycin cassette under a prokaryotic EM7 promoter. B. Copy number analysis by means of LightCycler PCR revealed that Advillin-Cre mice have five copies per genome.