Figure 3

X-gal staining in tissue sections from adult mice. Cryo-sections from adult tissue after staining with X-gal, NeuN, or GFP. A. DRG. B. circumvallate taste buds in the tongue. C. foliate taste buds in the tongue. D. trigeminal ganglion. E. sagittal brainstem section. F. close up of caudal brainstem with staining in spinal tract of trigeminal nerve and spinal nucleus of trigeminal nerve (arrow). G. close up of rostral brainstem region with some staining in the medial trigeminal nucleus (arrowhead) and medial vestibular nucleus (star). H. Midbrain section with diffuse staining in the ventral tegmental area. I. coronal brainstem section with staining in secondary sensory ganglia. J. SCG section. K. and L. DRG sections with NeuN staining (red) and GFP staining (green) for quantification. M. DRG section with X-gal staining illustrating the quantification procedure. Scale bar: A. and D. 10 micrometer. B., C., F., G., J., K., L., and M. 100 micrometer. E., H., and I. 1 mm.