Complete relief from ICS-induced mechanical allodynia. Basal mechanical paw-withdrawal threshold (PWT, in grams) was assessed at P14, using paw pressure tests. Intrathecal injection of milnacipran (0.1 μg), amitriptyline (15 μg), mianserin (20 μg), or paroxetine (5 μg), was given once daily from P1-P5, as described in Figure 4. *p < 0.05, vs. vehicle-treated control group; #p < 0.05, vs. vehicle-treated and ICS-exposed groups. Data are expressed as the means ± S.E.M.; 3-6 mice per group.