Table 3 Quantification of western blots on ipsilateral vs. contralateral sciatic nerves from SNL rats 17 days post-surgery.
RAT SNL | |||||
|---|---|---|---|---|---|
Sciatic Nerve Ipsilateral vs. Contralateral | |||||
| Â | Contra | Contra | Ipsi | Ipsi | Â |
Protein/Standardization | Mean % | SEM | Mean % | SEM | P-value |
p-mTOR/mTOR | 100 | 27.6 | 581.8 | 112.0 | 0.002 |
p-4EBP/4EBP | 100 | 8.9 | 117.0 | 13.6 | NS |
p-4EBP/4EBP | 100 | 12.6 | 408.0 | 104.2 | 0.008 |
p-rS6/beta III-tubulin | 100 | 22.6 | 1005.5 | 300.0 | 0.007 |
p-AKT/AKT | 100 | 17.3 | 101.6 | 22.0 | NS |
p-AKT/beta III-tubulin | 100 | 14.4 | 276.4 | 67.5 | 0.034 |
AKT/beta III-tubulin | 100 | 11.2 | 261.6 | 36.9 | 0.003 |
p-ERK/ERK | 100 | 8.8 | 510.3 | 153.4 | 0.023 |
p-eIF4E/eIF4E | 100 | 14.8 | 164.0 | 17.6 | 0.024 |
p-eIF4E/beta III-tubulin | 100 | 25.1 | 700.8 | 168.5 | 0.008 |
eIF4E/beta III-tubulin | 100 | 16.8 | 415.4 | 62.7 | 0.001 |
eIF4A/beta III-tubulin | 100 | 14.6 | 241.0 | 31.0 | 0.001 |
p-eIF2alpha/eIF2alpha | 100 | 8.9 | 110.7 | 21.4 | NS |
p-eIF2alpha/beta III-tubulin | 100 | 15.3 | 506.4 | 163.4 | 0.024 |
eIF2alpha/beta III-tubulin | 100 | 19.5 | 575.7 | 107.9 | 0.001 |
FMRP/beta III-tubulin | 100 | 14.2 | 257.8 | 59.0 | 0.024 |
mov10/beta III-tubulin | 100 | 28.5 | 636.3 | 161.2 | 0.008 |
rck/beta III-tubulin | 100 | 22.9 | 483.4 | 141.4 | 0.015 |
fbxw7/beta III-tubulin | 100 | 37.7 | 305.7 | 72.9 | 0.028 |
p-4EBP/GAPDH | 100 | 25.4 | 224.6 | 18.6 | 0.004 |
4EBP/GAPDH | 100 | 26.1 | 257.4 | 32.5 | 0.006 |
p-eIF4E/GAPDH | 100 | 15.9 | 458.0 | 54.4 | 0.000 |
eIF4E/GAPDH | 100 | 10.6 | 270.2 | 12.4 | 0.000 |
p-eIF2alpha/GAPDH | 100 | 10.3 | 297.4 | 74.9 | 0.018 |
eIF2alpha/GAPDH | 100 | 4.7 | 244.3 | 15.7 | 0.000 |
rck/GAPDH | 100 | 20.5 | 280.0 | 62.2 | 0.013 |
p-AKT/GAPDH | 100 | 8.4 | 182.8 | 26.1 | 0.017 |
AKT/GAPDH | 100 | 14.1 | 177.2 | 18.6 | 0.011 |
eIF4A/GAPDH | 100 | 9.3 | 249.6 | 33.0 | 0.001 |
fbxw7/GAPDH | 100 | 25.4 | 420.6 | 134.1 | 0.037 |