Figure 4From: Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury Characterization of TLR2 and CCL2 expression in DRG cells. (A) CCL2 is expressed in DRG neurons after nerve injury. DRGs of un-injured WT mice (WT Sham; a, b, and c), injured WT mice at 1 dpi (WT; d, e, and f), and injured TLR2 knock-out mice (TLR2 KO; g, h, and i) at 1 dpi were immunostained with anti-NeuN antibody (shown in red, a, d, and g) and anti-CCL2/MCP-1 antibody (shown in green, b, e, and h). Merged images are shown in the right panels. (B) TLR2 immunoreactivity (red color in a, d, and g) merged with GFAP-IR satellite cells (e) in DRG at 7 dpi, and to Iba-1-IR macrophages (h) in un-injured DRGs, but not to MAP2-IR neurons (b). Merged images are shown in the right panels (c, f, and i). The triangles indicate double-positive cells. Scale bars: 50 μm.Back to article page