PPD and VGAT immunoreactivity in lamina I. a, b: Confocal images scanned to reveal PPD (green) and VGAT (magenta). c: a merged image. Many VGAT boutons are visible throughout the field, and 3 of these (arrows) are also PPD-immunoreactive. The asterisk in a and c indicates part of a PPD+ cell body. The images are projections from 3 optical sections at 0.3 μm z-separation. Scale bar = 5 μm.