Co-localisation of PPD and galanin in GABAergic boutons. a-c: Confocal images scanned to reveal PPD (red), galanin (Gal, green) and VGAT (blue). d: a merged image. This field from lamina IIo contains two boutons (arrows) that are immunoreactive with all 3 antibodies. Note that several boutons that are immunoreactive only for galanin are also present, and these are likely to belong to peptidergic primary afferent axons, which also contain galanin. The images are from a single optical section. Scale bar = 5 μm.