Patch clamp recording from WT and KI cultured neurons. A, Representative current traces evoked by α,β-meATP (10 μM, 2 s) from WT and KI neurons in control and after MβCD pre-treatment (30 min, 10 mM). B, Histograms quantify the current amplitude before or after MβCD pre-treatment for WT and KI neurons. Control: n = 33 for WT and KI. *: p = 0.006. **:p = 0.008; ***: p = 0.002. MβCD: n = 30 for WT and KI. C, P2X3 mediated current traces (average of 14-20 responses) in control (black) and after MβCD pre-treatment (gray), normalized and overlapped, from WT (left) and KI (right) neurons. Note no change in the current onset, and clear acceleration of its slow decay.