Figure 4

Properties of P2X3 receptor mediated responses of WT and KI neurons. Histograms quantify the different properties of P2X3 receptors in WT (left) or KI (right) trigeminal neurons in control and after MβCD treatments. Note that MβCD treatment accelerates the slow desensitization time constant and slows down the degree of recovery from desensitization in WT and KI neurons (tested at 30 s interval). Data refer to effects induced by 10 μM α,β-meATP. A, current onset expressed as 10-90% response rise time. Control: n = 20 for WT and n = 25 for KI. MβCD: n = 15, p > 0.05 for WT; n = 27, p > 0.05 for KI. B, desensitization onset (fast desensitization time constant, τ_fast) Control: n = 18 for WT and n = 20 for KI. MβCD: n = 12, p > 0.05 for WT and n = 24, p > 0.05 for KI. C, retarded current decay (slow desensitization time constant, τ_slow). Control: n = 18 for WT and n = 20 for KI. MβCD: n = 11, p < 0.01 for WT and n = 21, p = 0.04 for KI. D, recovery from desensitization, expressed as % of control response amplitude with 30 s paired pulse protocol of α,β-meATP application. Control: n = 12 for WT and n = 16 for KI. MβCD: n = 7, p = 0.03 for WT and n = 11, p < 0.01 for KI.