Figure 3
From: Soluble epoxide hydrolase limits mechanical hyperalgesia during inflammation
Consequences of sEH deletion on EET and prostaglandin levels in the inflamed paw. (A-C) Changes in EET levels during zymosan induced inflammation. Levels of 8,9- (A), 11,12-(B) and 14,15-EET (C) levels were determined by LC-MS/MS in paw tissues at different time points after zymosan injection. (D) Changes in EET and DHET (E) levels after sEH deletion. Four different regioisomers were determined by LC-MS/MS analysis in paw tissue from wild type and sEH-/- mice 48 h after intraplantar zymosan injection. Data shown represent the mean ± SEM from tissues of 4-5 animals. Student's t test: *, p ≤ 0.05. (F) Effect of sEH deletion on COX-2 expression. COX-2 protein levels were determined by western blot in paw tissues from wild- type and sEH deficient mice 48 h after intraplantar zymosan injection. (G) Effect of sEH deletion on prostaglandin synthesis in the inflamed paw. Prostaglandins were determined 48h after zymosan injection by LC-MS/MS analysis. Data shown represent the mean ± SEM from tissues of 3-5 animals. (H) Effect of sEH deletion on prostaglandin synthesis in the spinal cord. Prostaglandins were determined from lumbal spinal cord tissue 48h after zymosan injection. Data shown represent the mean ± SEM from tissues of 4 animals.