AMP hydrolytic activity is reduced in DRG and spinal cord of Pap-/-
, and dKO mice. Lumbar (A-D, I-L) DRG and (E-H, M-P) spinal cord sections from WT, Pap-/-
, and dKO mice were stained using AMP histochemistry at pH 7.0 or pH 5.6. Arrows (H and P) point to lamina II. Staining intensity in lamina II was quantified from 7-10 sections per genotype as described (Methods) and reported (bottom left) as means ± s.e.m. Repeated measures one-way ANOVA and Bonferroni's post-hoc tests were used to compare staining intensity between genotypes. At pH 7.0, staining intensity from Nt5e-/- and dKO slices were significantly decreased from WT (P < 0.0005), and at pH 5.6, Pap-/-
, Nt5e-/- and dKO were all significantly decreased from WT (Pap-/- and Nt5e-/-, P < 0.05; dKO, P < 0.0005). AMP concentration was 1 mM for DRG and 6 mM for spinal cord sections. Similar results were obtained from n = 3 mice of each genotype. Scale bars: 50 μm (D and L), 200 μm (H and P).