Figure 5

Spontaneous adenosine transients in lamina II are reduced in frequency in Pap^-/- , Nt5e^-/- and dKO mice. (A) Representative traces from each genotype showing adenosine concentration versus time, calculated from FSCV currents measured at 1.5 V. Traces were background subtracted every 60 s and baseline corrected to compensate for electrode drift over time (which creates a visible 60 s oscillation in the baseline). Raster plots below each trace mark each adenosine transient. Adenosine transients were considered to be events if the peak at 1.0 V was more than five times the standard deviation of the noise. (B) Adenosine transient frequency in WT (0.35 ± 0.04 events/min; n = 19 slices), Pap^-/- (0.14 ± 0.04 events/min; n = 10 slices), Nt5e^-/- (0.14 ± 0.07 events/min; n = 10 slices), and dKO (0.11 ± 0.05 events/min; n = 16 slices) mice. One-way ANOVAs with Bonferroni's post-hoc tests were used to compare each genotype to WT and to compare between genotypes. *P < 0.05, **P < 0.005. (C) Baseline fEPSP amplitude in WT, Pap^-/-, Nt5e^-/-, and dKO mice (n = 5/genotype). There were no significant differences between WT and mutant mice. Data presented as means ± s.e.m.