A nucleoside transport inhibitor promotes the buildup of ectonucleotidase-generated adenosine. (A) AMP was pressure ejected into lamina II of WT spinal cord slices in the absence or presence of DIP. FSCV was used to measure extracellular adenosine. (B) Average responses ± s.e.m., from n = 3 slices. DIP significantly increased the concentration of extracellular adenosine from 0.7 to 8 s following AMP addition (P < 0.005 by one-way ANOVA).