Figure 7
From: PAP and NT5E inhibit nociceptive neurotransmission by rapidly hydrolyzing nucleotides to adenosine
Dipyridamole, an adenosine transport blocker, gradually inhibits excitatory synaptic transmission in lamina II of WT mice but not A 1 R or dKO mice. (A) Illustration depicting the placement of the suction electrode to stimulate fEPSPs and the recording electrode in lamina II. (B1-B2) Representative Aδ fEPSPs in WT and A 1 R-/- spinal cord slices before (BL; black) and 25 min after (red) addition of DIP (25 μM) to the perfusate. (B3) Normalized fEPSP amplitude in WT and A 1 R-/- spinal cord slices (n = 15, and 14 respectively, P < 0.001 by two-way ANOVA and Bonferroni's post-hoc tests). (C1-C3) Representative Aδ fEPSPs in Pap-/- , Nt5e-/-, and dKO spinal cord slices before (black) and after (red) addition of DIP (25 μM). (C4) Normalized fEPSP amplitude in Pap-/- , Nt5e-/- , and dKO spinal cord slices (n = 13, 12 and 16 respectively). The normalized fEPSP amplitude after DIP addition was significantly larger in dKO slices when compared to WT (P < 0.001 by two-way ANOVA and Bonferroni's post-hoc tests). There were no significant differences between WT and Pap-/- or WT and Nt5e-/- fEPSP amplitudes after DIP addition. (B3, C4) Data are presented as means ± s.e.m.