Beclin 1 expression in the spinal cord following spinal nerve ligation. (A) Beclin 1 expression was analyzed by Western blot in the hemi-cord contralateral (C) and ipsilateral (I) to the side of ligation, 7 days after surgery. SNL animals showed higher Beclin 1 expression than sham animals (A). A clear trend towards an increase in Beclin 1 expression in the ipsilateral (I) compared to the contralateral (C) side was observed after ligation (A) and confirmed by densitometric analysis (B). The slight increase in Beclin 1 expression well correlated with α2δ-1 upregulation (A and C). Signals from each band were normalized towards the corresponding GAPDH signal. One representative Western blot is shown. Sham: n = 5, SNL: n = 6.