Figure 2

Electrophysiological characterization of CA1 pyramidal neurons from wild type and transgenic mice. A. Representative deactivation currents recorded from CA1 pyramidal neurons in fresh slice preparations from wild type (WT, left panel) or transgenic mice (Tg, right panel). Overlay of currents evoked by the protocol in the absence (black) or presence (red) of 10uM XE991. B. Response of CA1 pyramidal neurons with injection of 1000ms, 30pA depolarizing current pulse into the cell from WT (left panels) and Tg mice (right panels) before (black) and after (red) application of XE991. XE991 increased action potential firing in WT mice, but it was less effective in neurons from Tg mice. C. Comparison of average number of spikes evoked by injected current in WT and Tg neurons. D-F. Box plots illustrating the differences in WT and Tg mice in resting membrane potential, threshold current required for action potential firing and input resistance, n = 10; Data are shown as mean ± s.e.m., *p < 0.05.