Intraplantar injection of kisspeptin amplified the increase in TRPV1 phosphorylation in the skin of mice treated with formalin. A representative immunoblot of (Ser800)-phosphorylated TRPV1 in the skin of naïve mice and mice injected with formalin in the absence or presence of kisspeptin (3 nmol/5 μl) is shown in (A). Densitometric analysis is shown in (B), where values are means + S.E.M. of 4 determinations. *p < 0.05 vs. naïve mice, #p < 0.05 or vs. mice treated with formalin alone (one-way ANOVA followed by Fisher's post hoc test).