Expression of COX-1, COX-2 and Egr-1 mRNA in spinal cord. Real-time semi-quantitative measurement of COX-1 (a), COX-2 (b) and Egr-1 (c) mRNA in spinal cord from control sheep (n = 6) and sheep affected by unilateral lameness on day 0 (pre-treatment) (n = 6) and 3 days post-treatment (n = 6). Spinal cords were hemisected into ipsilateral and contralateral portions (data is pooled for control animals). Levels of mRNA are expressed relative to the endogenous reference gene β-actin. Significant increase from control: * p < 0.05.