Figure 2From: Cannabinoid CB2 Receptors Contribute to Upregulation of β-endorphin in Inflamed Skin Tissues by Electroacupuncture Effects of AM1241, EA, AM1241 plus AM630, and EA plus AM630 on the mRNA level of POMC (A, B) and the protein level of β-endorphin (C, D, E) in inflamed skin tissues. A, summary data show the relative mRNA level of POMC in the skin tissues obtained from the vehicle control (CONT), CFA+vehicle of AM1241 (CFA), CFA+AM1241 (AM1241), and CFA+AM1241+AM630 (AM1241+AM630) groups. B, summary data show the relative mRNA level of POMC in the skin tissues obtained from the CFA+sham EA+vehicle of AM630 (sham EA), CFA+EA+vehicle of AM630 (EA), and CFA+EA+AM630 (EA+AM630) groups. C, a representative gel image showing the protein level of β-endorphin in the skin tissues obtained from 7 groups of rats. β-actin was used as a loading control. The protein band at 3.5 kDa corresponds to the β-endorphin protein. D-E, summary data show the % increase in the β-endorphin protein level by AM1241 and EA with and without AM630. Data are expressed as means ± SEM (n = 6 rats in each group). * P < 0.05, compared with the vehicle control group; # P < 0.05, compared with the CFA+vehicle of AM1241 group; & P < 0.05, compared with the CFA+AM1241 group; †P < 0.05, compared with the CFA+sham EA+vehicle of AM630 group; $ P < 0.05, compared with the CFA+EA+vehicle of AM630 group (One-way ANOVA followed by Tukey's test).Back to article page