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Figure 2 | Molecular Pain

Figure 2

From: Neurotrophic factor changes in the rat thick skin following chronic constriction injury of the sciatic nerve

Figure 2

Changes in p75 expression on S100-IR Schwann cells. A) In sham-operated rats Schwann cells (green) were detected by means of S100 immunoreactivity in small nerves, with immunoreactivity of varying intensity from bright (arrow), lower in the dermis, to dim, along the dermo-epidermal junction. P75 immunoreactivity (red) was evident surrounding all S100-IR Schwann cells. B) One week following nerve injury, p75 immunostaining was dramatically upregulated in Schwann cells; the intense red color masked the mixture of red and green stainings which could be detected by analysing the separately the S100 and p75 stainings (not shown). C-D) 2 & 4 weeks post-injury a decrease in p75 intensity was observed in that the yellow indicative of S100 co-labelling was able to be visualized. E) proNGF (red) S100 (green) and p75 (blue) triple labelling to demonstrate the relative distribution of Schwann cells with respect to proNGF; note a limited distribution of Schwann cells with proNGF and faint immunoreactivity for p75 in sham-operated controls. F) 2 weeks post-injury, the clear upregulation of p75 immunoreactivity associated with S100 (arrow) was observed, which wrapped around proNGF-IR blood vessels.

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