Skip to main content

Table 1 Primers sequence for RT-qPCR and results of RT-qPCR analysis.

From: The expression of spinal methyl-CpG-binding protein 2, DNA methyltransferases and histone deacetylases is modulated in persistent pain states

mRNA

 

Primers sequence

CFA

SNI

   

Ispi

vs Sham

Contra

vs Sham

Ispi

vs Sham

Contra

vs Sham

MeCP2 e1

F

5'-ggagagactggaggaaaagtca-3'

137% **

158% *

74% *

ns

 

R

5'-ccttcttaaacttcaggggtttc-3'

    

MeCP2 e2

F

5'-ctgtttgggagaagcagagg-3'

ns

134% *

85% *

ns

 

R

5'-tggtagctgggatgttaggg-3'

    

FKBP5

F

5'-caccctgagcctggagagag-3'

ns

ns

ns

ns

 

R

5'-gtgtcgccattactcgcagag-3'

    

SGK1

F

5'-gggctgtcttgtatgagatgc-3'

ns

ns

ns

ns

 

R

5'-gtgccttgctgagttggtg-3'

    

SULT1a1

F

5'-ccgaggagactgtggattc-3'

ns

ns

ns

ns

 

R

5'-gcatagtgggcatcaaagc-3'

    

DNMT1

F

5'-ggagtgtgtgaaagagaaa-3'

112% *

114% **

87% *

ns

 

R

5'-tagccttcctcagacaat-3'

    

DNMT3a

F

5'-aattgtgtcttggtggat-3'

125% *

124% *

ns

ns

 

R

5'-actgagaatttgccatct-3'

    

DNMT3b

F

5'-ggagacagcagacatctt-3'

ns

131% *

ns

ns

 

R

5'-gtcactacagttcccattaac-3'

    

HDAC1

F

5'-cctcaccgaatccgaatg-3'

120% *

128% **

ns

ns

 

R

5'-cgaatagaacgcaagaacttg-3'

    

HDAC2

F

5'-tcaagtttctacgatcaataagg-3'

ns

ns

ns

90% *

 

R

5'-cttctccgacattaaatctctg-3'

    

HDAC5

F

5'-aagtacgttcaaggctaa-3'

117% **

114% **

77% ***

ns

 

R

5'-cgactgctctcttcttaa-3'

    

β-Actin

F

5'-agattactgccctggctccta-3'

ns

ns

ns

ns

 

R

5'-aggatagagccaccaatccac-3'

    

HGPRT

F

5'-aggacctctcgaagtgttggatac-3'

ns

ns

ns

ns

 

R

5'-tgtagattcaacttgccgctgtc-3'

    
  1. * P < 0.05; ** P < 0.01; ns: non significant. CFA N = 5 in each group and SNI N = 7 in each group