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Figure 4 | Molecular Pain

Figure 4

From: Warmth suppresses and desensitizes damage-sensing ion channel TRPA1

Figure 4

Effects of warming on acute desensitization of TRPA1. A, an example of the effects of heat following two consecutive application of MO (30 μ M) in a HEK293 cell transiently transfected by rat TRPA1. Scale bar, 3 min, 1 nA. B, representative current traces demonstrating the effects of temperatures on desensitization of rat TRPA1 following MO-evoked activation at 23°C. Currents were obtained from HEK293 cells stably expressing rat TRPA1. Washout of MO was performed at 23°C (left) or 39°C (right). The changes in temperature during recordings are shown in lower panels. Vertical dotted lines represent equivalent duration of time following washout of MO in different temperature as indicated. C, Examples of relative current traces recorded during the period between dotted lines in panel B obtained from multiple cells. The arrow indicates the time point at which the exposure to different temperatures was initiated. D, decay τ measured from the experiments in panel B in the presence of extracellular Ca2+ or Ba2+ at 23 (white) or 39°C (black). *, P < 0.05; **, P < 0.005 in Student's t test; n = 4-6.

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