Effects of intrathecal (i.t.) injection of tetrodotoxin (TTX) on pain behaviors and motor function in rats with intra-tibial injections of MRMT-1 cells. (A): 50% paw withdrawal threshold (PWT); (B): paw withdrawal latency (PWL); (C): inclined-plate test. Note that i.t. injection of TTX inhibited the tumor-evoked (as assessed by 50% PWT) and thermal hyperalgesia (as assessed by PWL), but did not affect the motor function in bone cancer rats. **p < 0.01, ***p < 0.001, as compared with i.t. injection of saline rats, two-way ANOVA followed by Bonferroni post-hoc test.