Figure 1

A: Temporal profile of the microglial cell activation in the prV and motV. In the case of IAN-X rats, microglial cells were immuno-labeled with Iba1 in the prV and motV at 3 (n = 5) and 14 day (n = 5) after IAN-X. In the sham (n = 5) or IAN-X + MC-injected (both the i.p., n = 5 and m.i., n = 5) or IAN-X + saline-injected rats (i.p., n = 5 and m.i., n = 5), microglial cells were immuno-labeled after 14 days. B: Localization of the m.i. site in the motV. The number of Iba1-immunoreactive (IR) cells in the three sections per rats (C-D). The Iba1-IR cells were increased at 3 day after IAN-X both in the prV (C) and motV (D). After 14 days, a small decrease in these cells was observed in IAN-X rats. The i.p. administration of MC strongly attenuated the number of Iba1-IR cells both in the prV and motV. It is notable that m.i. into motV strongly attenuated the number of Iba1-IR cell in the motV but not in the prV. (*) denotes the significant difference between experimental groups (p < 0.05, one way ANOVA with tukey test).