Figure 6From: The effect of minocycline on the masticatory movements following the inferior alveolar nerve transection in freely moving rats The time course of the present experiment. The rats were trained for behavioral testing at least 7 days before any surgical operation and drug or chemical injection. After the training, the rats were prepared for the sham operation or IAN-X, and the behavioral test was conducted for 14 days after the sham operation or IAN-X. In MC (both i.p. and m.i.) or saline (both i.p. and m.i.) injected rats, the drug was injected 16 h before transection, and was administrated once in a daily for 14 days after IAN-X. After behavioral test, the rats were prepared either for a second surgery prior to recording masticatory jaw movement or for perfusion prior to immunohistochemistry. In the case of IAN-X rats, the perfusion was conducted at 3 and 14 day after IAN-X. Note that the masticatory jaw movement was recorded for at least 4 days.Back to article page