Figure 5
From: Retracted: Comparison of central versus peripheral delivery of pregabalin in neuropathic pain states
Protein Quantification. Western blotting identified increased levels of CaVα2δ-1 protein in the dorsal horn and lumbar DRGs for rats exposed to either diabetic peripheral neuropathy (DPN) or spinal nerve ligation (SNL) when compared to control rats (A). In the dorsal spinal cord, either intrathecal or intranasal delivery of high dose (2.04 mg/kg/d) pregabalin was associated with less upregulation of CaVα2δ-1 protein than with saline delivery (B). The near nerve delivery of pregabalin failed to impact upon CaVα2δ-1 protein expression in the dorsal spinal cord following SNL or DPN (A, B). Finally, pregabalin delivered in any manner was not associated with impact upon CaVα2δ-1 protein expression in the lumbar DRGs exposed to either DPN or SNL when compared to saline delivery (intrathecal delivery results shown) (A). Multiple unmatched ANOVA tests were performed between cohorts receiving pregabalin and saline for each intervention and condition, with * indicating significant difference (p < 0.0125 after Bonferroni corrections) between cohorts. β-actin protein levels were used for quantification of ratios of CaVα2δ-1/β-actin demonstrated in B.