Recording in the ACC and S1 in vivo and calculation of the neuronal avalanche. (A) The recording site of the Michigan probe was placed in the ACC at an angle. The electrode tracks were visible as red Dil florescence that overlaid Nissl-stained histology. (B) The recording probe in the S1 was inserted vertically. The electrode tracks were visible as red Dil florescence that overlaid Nissl-stained histology. (C) Eight recording traces in four tracks are displayed in a typical example recorded in the ACC and S1, respectively. (D) The original electrophysiological recording was filtered to preserve high-frequency unit activity. The time-point was selected as the nLFP that exceeded four-times the standard deviation of the threshold of basal activity. (E) The nLFP time-points were counted from each channel and framed in 4 ms time bins. Each avalanche event is defined as the continuous selective nLFP response until a blank frame occurred. The involved channel numbers in each avalanche event were summed as the avalanche size. (F) The distribution of the avalanche size with occurrence probability is plotted on a log-log scale. A neuronal avalanche that has a fitted straight-line slope of the α value indicates a power law relationship. Shuffled data were calculated from raw recording data.