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Figure 5 | Molecular Pain

Figure 5

From: Mitogen activated protein kinase phosphatase-1 prevents the development of tactile sensitivity in a rodent model of neuropathic pain

Figure 5

Effects of in vivo induction of spinal MKP-1 in behavioral hypersensitivity and spinal p-p38 expression. Withdrawal threshold to mechanical stimulation (a) in rats undergone L5 nerve transection surgery and treated i.t. with PEI only (n = 9), PEI + empty cDNA (n = 4), and PEI + MKP-1 cDNA (n = 9) four days after treatment and surgery. Quantification of p-p38 protein (b) and representative Western blot of p-p38 (c) expression from L5–L6 spinal cord of rats that have undergone L5 nerve transection surgery and been treated i.t. with PEI only (n = 3), PEI + empty cDNA (n = 3) and PEI + MKP-1 cDNA (n = 3) four days after treatment and surgery. *P < 0.05 versus PEI only or PEI + empty vector. All data are expressed as mean ± s.e.m. (d) Representative images obtained by confocal microscopy and immunofluorescence depicting p-p38 (in green) and the microglial marker Iba-1 (in red), the neuronal marker NeuN (in red), and the astrocytic marker S100b (in red) in spinal cord of rats four days after L5 nerve transection and treated with PEI + MKP-1 cDNA or PEI only. Arrows indicate co-regionalization (in yellow/orange).

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Molecular Pain

ISSN: 1744-8069

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