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Figure 5 | Molecular Pain

Figure 5

From: Inflammation-induced changes in BKCa currents in cutaneous dorsal root ganglion neurons from the adult rat

Figure 5

Inflammation was associated with no detectable change in total BK Ca channel subunit protein levels. Total protein was extracted from L4 and L5 ganglia from naïve and inflamed rats ipsilateral (I) or contralateral (C) to the site of CFA injection. A. Example of a western blot of the BKCa α subunit. A sample of total protein from the uterus of a naïve rat was used as a loading control to enable comparisons between blots. Ispilateral and contralateral in the naïve rats were simply left and right, respectively, as all CFA injections in the inflamed group were made in the left hindpaw. GAPDH was used as a loading control. B. Example of western blots for BKCa β subunits 2, 3, and 4. The uterus protein sample was again used to enable comparisons between blots. Pooled data for relative levels of α (C), and β (D) subunit revealed no significant differences between groups

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