Figure 3

A-C Spinal cords from Sap pre-treated animals. A shows dorsal horn from rat perfused 0.75 h post carrageenan, green = P-Akt, most of the P-Akt is in the superficial dorsal horn. Calibration mark = 100 μm and is the same for all dorsal horn pictures. The middle panel (B) shows dorsal horn from an animal perfused 2.0 h post carrageenan. Note that P-Akt activity has shifted from the superficial to the deeper laminae. The bottom panel (C) shows P-Akt in ventral horn 0.75 h post-carrageenan. Calibration mark = 50 μ. Pretreatment with SSP-Sap reduces carrageenan-induced P-Akt in superficial dorsal horn (D) and ventral horn (F) at 0.75 h and in laminae IV and V at 2 h (E). G Mean number of P-Akt positive neurons ± SEM counted in each area under the two conditions, Sap and SSP-Sap pretreatment. Cells were counted only if the P-Akt was co-localized with NeuN (not shown). * p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001 Pictures (A-F) and counts (G) are from sides ipsilateral to the injection.