Figure 2

Change in Na⁺ channel transcription on L4-5 DRG, as measured by real-time RT-PCR. Elcatonin (15 U/kg), given subcutaneously five times a week from 11 to 26 or 27 days post-surgery, improved the CCI-induced change in Na⁺ channel transcription on ipsilateral L4-5 DRG. Effect of eCT treatment on (a) Nav1.3, (b) Nav1.7, (c) Nav1.8 and (d) Nav 1.9 expression normalized to the endogenous control GAPDH. (e) Elcatonin did not affect the gene expression of Na⁺ channels in contralateral intact DRG. The relative amount of target was quantitated by the relative standard curve method (mean in contralateral intact-vehicle group = 1). Each value represents the mean ± SEM(n = 12). ANOVA revealed (a, c, d) the significant differences (P < 0.01) and (b) no difference (P = 0.3049). Significant differences by Dunnett’s test: **P < 0.01,*P < 0.05, as compared to the CCI-vehicle.