Figure 3

Changes in expression of calcitonin receptors (CTR) after surgery on the sciatic nerve. (a) The time course of change in CTR mRNA expression in the CCI site. (b) The CTR gene expression was slightly influenced in sham operation compared to contralateral sciatic nerve in CCI rats. (c) The additional downregulation of CTR mRNA on CCI site by five injections of eCT (20 U/kg/day) immediately following CCI. (d) Elcatonin (20 U/kg), injected subcutaneously five times a week from 11 to 26 days post-surgery, suppressed the CCI-induced increase in CTR gene expression to the intact level. CTR gene expression was normalized to the endogenous control RPL19. The relative amount of target was quantitated by the relative standard curve method (mean in intact-vehicle group at day 1 = 1). Each value represents the mean of 6 data points (12 animals) ± SEM. (a)(b) Two-way or (c) one-way ANOVA exhibited the significant differences (P < 0.01). Significant differences by t-test or Dunnett’s test: **P < 0.01,*P < 0.05, as compared to the CCI-vehicle.