Figure 4
Analysis of125 I-calcitonin binding site in the sciatic nerve membrane. (a) Saturation analysis of 125I-CT binding to mixed membranes taken from 15 sciatic nerves in sham rats. (b) Saturation analysis of 125I-CT binding to mixed membranes taken from 15 sciatic nerves with the CCI regions. Scatchard analysis of specific binding data from (a) or (b) were presented in insert plots, respectively. (c) CCI decreased in density of 125I-CT (0.2 nM) binding site in the sciatic nerve membrane 3 days after the operation. (d) CCI increased the density of 125I-CT (0.2 nM) binding sites in the sciatic nerve membrane at 26 days post-surgery. Elcatonin (20 U/kg), injected subcutaneously five times a week from 11 to 26 days post-surgery, decreased in the density of 125I-CT binding site on the ipsilateral side to the sham level. (c) (d): Each value represents the mean of 6 data points from 12 animals ± SEM. One-way ANOVA exhibited the significant differences (P < 0.01). Significant differences by Dunnett’s test: **P < 0.01, as compared to (c) the sham or (d) the CCI-vehicle.