Figure 6

Changes in mRNA expression of CTR and Na⁺ channels at 5 days after nerve injury. Changes in CTR mRNA expression on ipsilateral sciatic nerve (a), spinal cord (b) or hypothalamus (c) (Experiment 1, 5 days post-surgery). It was verified that the specific downregulation of CTR mRNA on CCI segments from sciatic nerve in CCI rats and much further reduction in CCI-eCT rats. (d)(e)(f): Disappearance (offset) of eCT-induced normalization for Na⁺ channel gene expression on ipsilateral L4-5 DRGs in CCI rats (Experiment 1, 5 days post-surgery). (d), Nav1.3; (e), Nav1.8; (f), Nav1.9. Each value represents the mean ± SEM. ANOVA revealed (a, d, e) the significant differences (P < 0.01, P < 0.01, P = 0.0113) and (b, c, f) no difference (P = 0.6054, 0.6115, 0.0742). Significant differences by Dunnett’s test: **P < 0.01,*P < 0.05, as compared to the CCI-vehicle.