Figure 6
Ca2+ signals induced by puff-application of ATP. Ca2+ signals induced by puff-application of ATP. A, Representative example of Ca2+ signals in the spinal dorsal horn of a rat subjected to peripheral nerve injury (PNI) and treated with puff-application of ATP. White dots in the left image indicate cells in the spinal dorsal horn loaded with Fluo-4/AM during preapplication of ATP. The right image shows the Ca2+ signal response to puff-application of ATP 10 s after application. B, Quantification of the number of cells showing a Ca2+ signal response to ATP in control rats, rats injected with complete Freund adjuvant (CFA), and rats subjected to PNI. *P < 0.05, **P < 0.01 compared to control rats. C, The upper graph indicates the effect of the microglial inhibitor minocycline (20 μM), the specific P2X1,2,3,5 antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS, 20 μM), the specific P2X1,2,3,4 antagonist 2',3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate (TNP-ATP, 1 μM) on Ca2+ signals in rats subjected to PNI and responding to ATP. The lower graph indicates the effect of the microglial inhibitor minocycline (20 μM), the astroglial toxin L-alpha-aminoadipate (L-α-AA, 1 mM) and the specific P2X1,2,3,5 antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS, 20 μM) on Ca2+ signals in rats subjected to CFA and responding to ATP. Data are expressed as % of the decrease in the number of cells responding to drug application. *P < 0.05, **P < 0.01