Intraplantar carrageenan induces significant IgG extravasation in spinal cord. Intraplantar carrageenan induces the appearance of IgG in endothelial cells and nervous tissue at 72 hours post-carrageenan in both male (A) and female (D) rats. Quantification reveals significantly increased fluorescence intensity in both male (B) and female (E) rats at this timepoint, but not with dorsally-injected subcutaneous carrageenan, or with capsaicin. Significant IgG is also detected in the thoracic region which correspondingly also peaks at 72 hours post-carrageenan (C). High magnification picture and triple staining of occludin, VWF, and IgG are shown (F). Endothelial cells with IgG staining show intact occludin protein within a normal range (G). Abbreviations: Immunoglobulin (IgG), others as in Figure 1, 2, and 3.