No major histopathological changes, sodium fluorescein, or Evans Blue dye extravasation detected at 72 hours after intraplantar carrageenan. H&E staining (A) and Nissl staining (B) do not reveal major lumbar spinal cord structural abnormalities at 72 hours post-carrageenan as compared to vehicle. There is no significant detection of extravasated Evans Blue (C) or NaFL (D) dye in spinal cord or brain in neither male nor female rats at 72 hours post-carrageenan as compared to vehicle treatment. Intrathecal LPS injected animals do show significant leakage of dye in lumbar, thoracic, and cervical regions. Abbreviations: lumbar (L), thoracic (T), cervical (C), midbrain (MB), cerebral cortex (CC), others as in Figure 1, 2, 3, and 4.